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. 2020 Aug 19;21(17):5949. doi: 10.3390/ijms21175949

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Semen exosome miR-30d-5p, miR-93-5p and miR-449a Cp values obtained by RT-qPCR. RNA was obtained from extracellular vesicle extracts isolated by using different techniques: ExoGAG (3500× g), miRCURY Cell/Urine/CSF (1500× g) and Ultracentrifugation (100,000× g) methods. First-stranded cDNA specific for miRNA was obtained by RT of 50 µg in 10 µL using the Universal cDNA synthesis kit (Exiqon). The three semen exosomal miRNAs were amplified in a Lightcycler 96 instrument. No difference in the Cp values were found between the three methods. The values in the graph are the mean Cp values ± SD. RT-qPCR: quantitative reverse transcription PCR.