Table 3.
Summary of miRNA expression data in semen vesicles obtained with the three most efficient EV isolation methods.
| A. | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Average Cq ULTRACENTRIFUGATION | p-value | miRNA expression | p-value | ||||||
| Gene Name | HCt | BPH | PCa | CV | (HCt-BPH-PCa) | HCt | BPH | PCa | HCt-PCa |
| let7i-3p | 33.39 | 33.01 | 36.14 | 0.076 | 0.049 | 1 | 1.360 | 0.395 | 0.083 |
| miR 106b-5p | 24.15 | 23.00 | 24.64 | 0.075 | >0.10 | 1 | 1.905 | 1.083 | >0.10 |
| miR 125a-5p | 21.94 | 21.03 | 21.85 | 0.032 | >0.10 | 1 | 1.417 | 1.270 | 0.060 |
| miR 130a-3p | 28.41 | 26.96 | 28.09 | 0.042 | >0.10 | 1 | 1.986 | 1.466 | >0.10 |
| miR 142-3p | 29.51 | 27.10 | 28.19 | 0.056 | 0.059 | 1 | 5.309 | 3.066 | 0.042 |
| miR 142-5p | 31.30 | 29.93 | 31.43 | 0.060 | >0.10 | 1 | 2.291 | 1.443 | >0.10 |
| miR 181c-5p | 30.10 | 30.17 | 30.52 | 0.027 | >0.10 | 1 | 0.694 | 0.845 | >0.10 |
| miR 196b-3p | 29.03 | 29.63 | 30.14 | 0.024 | 0.014 | 1 | 0.498 | 0.562 | 0.004 |
| miR 223-3p | 29.54 | 27.02 | 29.69 | 0.105 | >0.10 | 1 | 7.742 | 2.627 | >0.10 |
| miR 30c-5p | 22.31 | 20.20 | 21.57 | 0.059 | 0.038 | 1 | 3.904 | 1.892 | 0.042 |
| miR 30e-3p | 25.45 | 23.22 | 24.75 | 0.047 | 0.007 | 1 | 4.139 | 1.746 | 0.060 |
| miR 34a-3p | 30.11 | 28.24 | 29.38 | 0.041 | 0.050 | 1 | 3.452 | 2.080 | 0.019 |
| miR 34a-5p | 25.46 | 24.40 | 25.52 | 0.037 | 0.027 | 1 | 1.682 | 1.172 | >0.10 |
| miR 576-5p | 30.66 | 29.78 | 30.65 | 0.026 | 0.055 | 1 | 1.348 | 1.134 | >0.10 |
| miR 663b | 28.03 | 29.03 | 29.68 | 0.045 | 0.078 | 1 | 0.318 | 0.267 | >0.10 |
| miR 92a-3p | 21.34 | 20.41 | 21.34 | 0.037 | 0.034 | 1 | 1.657 | 1.169 | >0.10 |
| miR (576+181) | 0.024 | ||||||||
| B. | |||||||||
| Average Cq miRCURY cell/urine/CSF | p -value | miRNA expression | p -value | ||||||
| Gene Name | HCt | BPH | PCa | CV | (HCt-BPH-PCa) | HCt | BPH | PCa | HCt-PCa |
| let7i-3p | 31.43 | 30.75 | 32.27 | 0.110 | >0.10 | 1 | 2.17 | 1.60 | >0.10 |
| miR 106b-5p | 22.81 | 22.86 | 23.32 | 0.050 | >0.10 | 1 | 0.82 | 0.79 | >0.10 |
| miR 125a-5p | 21.30 | 21.32 | 21.74 | 0.031 | >0.10 | 1 | 0.80 | 0.79 | >0.10 |
| miR 130a-3p | 26.78 | 25.38 | 26.18 | 0.041 | 0.076 | 1 | 2.66 | 1.62 | 0.060 |
| miR 142-3p | 28.34 | 26.69 | 27.19 | 0.069 | >0.10 | 1 | 3.26 | 2.44 | >0.10 |
| miR 142-5p | 30.16 | 28.45 | 29.02 | 0.058 | >0.10 | 1 | 3.52 | 2.41 | >0.10 |
| miR 181c-5p | 30.02 | 29.80 | 30.16 | 0.035 | >0.10 | 1 | 0.97 | 0.96 | >0.10 |
| miR 196b-3p | 28.76 | 28.87 | 28.86 | 0.027 | >0.10 | 1 | 0.77 | 1.04 | >0.10 |
| miR 223-3p | 28.81 | 27.21 | 28.52 | 0.072 | >0.10 | 1 | 2.01 | 0.90 | >0.10 |
| miR 30c-5p | 21.12 | 20.85 | 21.14 | 0.042 | >0.10 | 1 | 0.98 | 1.05 | >0.10 |
| miR 30e-3p | 24.75 | 24.34 | 24.82 | 0.037 | >0.10 | 1 | 1.04 | 1.02 | >0.10 |
| miR 34a-3p | 28.50 | 28.13 | 28.30 | 0.031 | >0.10 | 1 | 1.00 | 1.12 | >0.10 |
| miR 34a-5p | 24.84 | 24.63 | 25.00 | 0.036 | >0.10 | 1 | 0.97 | 0.91 | >0.10 |
| miR 576-5p | 29.91 | 29.60 | 29.90 | 0.029 | >0.10 | 1 | 1.03 | 1.05 | >0.10 |
| miR 663b | 28.33 | 26.97 | 27.19 | 0.043 | 0.077 | 1 | 2.15 | 4.53 | >0.10 |
| miR 92a-3p | 20.53 | 20.48 | 20.77 | 0.031 | >0.10 | 1 | 0.84 | 0.98 | >0.10 |
| miR (576+181) | 0.029 | ||||||||
| C. | |||||||||
| Average Cq EXOGAG | p -value | miRNA expression | p -value | ||||||
| Gene Name | HCt | BPH | PCa | CV | (HCt-BPH-PCa) | HCt | BPH | PCa | HCt-PCa |
| let7i-3p | 35.24 | 34.04 | 34.69 | 0.026 | 0.074 | 1 | 2.01 | 1.90 | >0.10 |
| miR 106b-5p | 25.25 | 25.09 | 25.34 | 0.043 | >0.10 | 1 | 1.04 | 1.17 | >0.10 |
| miR 125a-5p | 21.86 | 22.18 | 22.73 | 0.029 | 0.054 | 1 | 0.71 | 0.72 | 0.060 |
| miR 130a-3p | 28.74 | 27.42 | 28.25 | 0.035 | >0.10 | 1 | 2.03 | 1.69 | 0.083 |
| miR 142-3p | 31.23 | 28.99 | 29.88 | 0.057 | 0.038 | 1 | 5.05 | 3.34 | 0.042 |
| miR 142-5p | 32.27 | 30.78 | 31.72 | 0.043 | >0.10 | 1 | 2.59 | 1.93 | >0.10 |
| miR 181c-5p | 31.25 | 30.77 | 31.17 | 0.022 | >0.10 | 1 | 1.27 | 1.37 | >0.10 |
| miR 196b-3p | 29.19 | 29.87 | 29.37 | 0.031 | >0.10 | 1 | 0.55 | 1.05 | >0.10 |
| miR 223-3p | 29.08 | 27.94 | 28.41 | 0.053 | >0.10 | 1 | 3.31 | 1.83 | >0.10 |
| miR 30c-5p | 21.89 | 21.95 | 22.41 | 0.038 | >0.10 | 1 | 0.87 | 0.90 | >0.10 |
| miR 30e-3p | 26.58 | 26.66 | 27.04 | 0.039 | >0.10 | 1 | 0.93 | 0.96 | >0.10 |
| miR 34a-3p | 29.66 | 29.23 | 29.83 | 0.029 | >0.10 | 1 | 1.15 | 1.12 | >0.10 |
| miR 34a-5p | 26.91 | 26.50 | 27.17 | 0.044 | >0.10 | 1 | 1.13 | 1.11 | >0.10 |
| miR 576-5p | 30.18 | 30.33 | 30.96 | 0.028 | >0.10 | 1 | 0.81 | 0.73 | >0.10 |
| miR 663b | 24.53 | 24.46 | 24.55 | 0.043 | >0.10 | 1 | 0.65 | 1.25 | >0.10 |
| miR 92a-3p | 21.63 | 22.40 | 22.79 | 0.041 | 0.045 | 1 | 0.57 | 0.61 | 0.042 |
| miR (576+181) | 0.022 | ||||||||