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. 2020 Aug 20;21(17):6011. doi: 10.3390/ijms21176011

Figure 1.

Figure 1

Fas ligand (FasL) minigene construction: the FasL murine gene (7805 bp) consists of four exons (purple) and three introns (yellow). Untranslated regions (UTR—blue) flank the introns and the exons. FasL 5′ fragment (FasL fragment I) and FasL 3′ fragment (FasL fragment II) were amplified by PCR and successively cloned in pBluecript. The remaining fragments of the intron II were removed by overlapping PCR, and exons II and III (purple) were linked in frame. The obtained minigene (2887 bp) was cloned in the pAdTrack-CMV adenoviral vector in the KpnI/NotI restriction site.