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. 2020 Aug 20;21(17):6011. doi: 10.3390/ijms21176011

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FasL expression in transduced bovine aortic endothelial cells: Cells were transduced with increasing adenoviral titers in the range of 10–50 transduction units (TU) per cell. The cell surface expression of FasL protein was assessed using anti-CD95L antibodies (MFL4 clone) along with intrinsic fluorescence of green fluorescent protein (GFP). Effective induction of FasL expression was observed only for cells transduced with a vector encoding the minigene (Ad-gFasL), whereas only GFP expression was detected following transduction with a vector encoding cDNA (Ad-cFasL).