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. 2020 Sep 2;21(17):6391. doi: 10.3390/ijms21176391

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of knockdown PROK2 on cell viability and cell cycle in human cervical cancer HeLa cells. (A,B) Immunoblotting and RT-qPCR analysis of PROK2 protein and mRNA expression in three cervical cancer cell lines (C33A, HeLa and SiHa). β-actin as a protein loading control, GAPDH as a mRNA loading control. (C,D) The protein and mRNA expression of PROK2 in shLuc- or shPROK2-HeLa cells. (E) Cell viability of shLuc- or shPROK2-HeLa cells was measured by cell viability assay at 24 h and 48 h after seeding. (F) Cell cycle distribution of shLuc- or shPROK2-HeLa cells were measured by flow cytometry.

Effect of knockdown PROK2 on cell viability and cell cycle in human cervical cancer HeLa cells. (A,B) Immunoblotting and RT-qPCR analysis of PROK2 protein and mRNA expression in three cervical cancer cell lines (C33A, HeLa and SiHa). β-actin as a protein loading control, GAPDH as a mRNA loading control. (C,D) The protein and mRNA expression of PROK2 in shLuc- or shPROK2-HeLa cells. (E) Cell viability of shLuc- or shPROK2-HeLa cells was measured by cell viability assay at 24 h and 48 h after seeding. (F) Cell cycle distribution of shLuc- or shPROK2-HeLa cells were measured by flow cytometry.