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. 2020 Sep 2;21(17):6391. doi: 10.3390/ijms21176391

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MMP15 involved in PROK2 regulates cell migration and invasion in human cervical cancer HeLa cells. Using transfected with Neo or PROK2 overexpression plasmid in shLuc- or shPROK2-HeLa cells for 48 h. (A) The protein expression of MMP15 and PROK2 were measured by the western blotting. β-actin as a protein loading control. (B) The MMP15 and PROK2 mRNA expression were detected by RT-qPCR assay. GAPDH as a mRNA loading control. (C) In vitro migration and invasion assay was conducted to measures the cell migration and invasion numbers. ** p < 0.01 versus shLuc cells; # p < 0.05 verus shPROK2 cells.