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The effect of glaucoma and AAV-BDNF treatment on the retinal ganglion cell (RGC) number was examined by counting RGCs in 300 μm × 300 μm m fields in the central and peripheral parts of the retina (A). Images from non-treated eye (B) were compared to images from contralateral glaucomatous (C) or glaucomatous and AAV-BDNF treated eyes (D). RGCs were immunolabeled with anti-Brn3a antibody, a widely accepted marker of rodent RGC [31].
