Figure 4.

Inhibition of the classical pathway by selected hit compounds. All 24 hit fragments were tested for their ability to block C4 activation in an ELISA-based assay under conditions specific for the classical pathway. Each compound was tested in triplicate at a single concentration of 500 µM. Positive hits (four in total) were defined as any compound that significantly reduced C4b deposition relative to a non-binding control compound (CMP-685), as judged by an unpaired t-test (* p < 0.05).