Figure 3.

MMP is decreased after treatment with CD in T24 BC cells. Fluorescence staining of mitochondria by (A) Rh123 and (B) JC-1 at 200× and 400× magnifications. Scale bar = 50 µm. The representative photos were taken from one of the three independent experiments. Flow cytometry analysis of MMP by (C) Rh123 (D) JC-1 staining. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as a positive control for confirming the successful inhibition of MMP. Bar graphs show values normalised as percentages relative to the control. Data are expressed as mean ± SD of three (Rh123) and four (JC-1) independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 as compared with the control group (0 µM).