Figure 6.

The cumulative amount of AmB permeated per surface area (μg/cm²) through uninfected BALB/c mouse skin (n = 5) and L. major infected BALB/c mouse skin (n = 5). Infected skin was more permeable to both types of AmB-loaded chitosan nanoparticles than uninfected skin (p < 0.05 by using repeated measures ANOVA). The use of AmB-CH-TPP nanoparticles enhanced AmB penetration through both healthy and infected skin compared to AmB-CH-Dex nanoparticles (p < 0.05 by using repeated measures ANOVA). AmB-CH-TPP nanoparticles (Size = 65 ± 8 nm, Zeta potential = 25.5 ± 1 mV) or AmB-CH-Dex nanoparticles (Size = 170 ± 8 nm, Zeta potential = −13 ± 1 mV).