Table 1.
Physicochemical properties of blank and amphotericin B (AmB)-loaded chitosan nanoparticles.
| Nanoparticles | |||||
|---|---|---|---|---|---|
| CH-TPP | AmB-CH-TPP | CH-Dex | AmB-CH-Dex | ||
| EE % | after lyophilization with sucrose 5% | 94 ± 5 | 92 ± 8 | ||
| AmB loading % | after lyophilization with sucrose 5% | 26 ± 1 | 23 ± 2 | ||
| yield % | after lyophilization with sucrose 5% | 94 ± 4 | 93 ± 6 | 95 ± 3 | 92 ± 6 |
| size (diameter) nm | before lyophilization | 48 ± 6 | 57 ± 7 | 145 ± 6 | 164 ± 5 |
| after lyophilization with sucrose 5% | 67 ± 7 | 69 ± 8 | 170 ± 9 | 174 ± 8 | |
| PDI | Before lyophilization | 0.1 ± 0.01 | 0.1 ± 0.03 | 0.15 ± 0.01 | 0.16 ± 0.01 |
| after lyophilization with sucrose 5% | 0.25 ± 0.05 | 0.2 ± 0.01 | 0.29 ± 0.04 | 0.26 ± 0.01 | |
| zeta potential mV | before lyophilization | 32.1 ± 1.2 | 29 ± 2 | −15.5 ± 1 | −14 ± 2 |
| after lyophilization with sucrose 5% | 28.5 ±1.9 | 25.5 ± 1 | −12.9 ± 3 | −11 ± 1 | |
Data expressed as mean +/− SD (experiments were repeated three times and showed similar data). No significant difference was shown in % of EE, AmB loading and yield between AmB-CH-TPP and AmB-CH-Dex nanoparticles (p > 0.05 by using t-test). EE: encapsulation efficiency. PDI: polydispersity index.