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. 2020 Sep 20;17:276. doi: 10.1186/s12974-020-01912-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Assessment of ROS production and activation of NF-kB-p65. a) Percentage change showing levels of ROS in PDA post 24 h transfection with indicated plasmids (n=4). b) Percentage change showing the levels of total NF-kB-p65 in post transfected PDA (n = 5). c) Representative change in the expression of phosphorylated-NF-kB-p65 in all transfected PDA groups is represented in the graph (n=5). d) Representative western blot showing change in expression of NF-kB-p65 and phosphorylated NF-kB-p65 in transfected PDA (n=5). GAPDH was used as the loading control. e) Percentage change of the cytoplasmic fraction in PDA post 24 h transfection showing the levels of Nf-kB-p65 (n = 4). f) Expression levels of cytoplasmic Nf-kB-p65 were evaluated by represented western blots. GAPDH was used as the internal control (n=4). g) Percentage change of the nuclear fraction in PDA post 24h transfection showing the levels of Nf-kB-p65 (n=4). h) Expression levels of nuclear Nf-kB-p65 were evaluated by represented western blots.Total histone3 was used as the internal control (n =4). All data represent mean ± standard deviation from independent experiments (n stands for number of independent experiment), *p< 0.05, with respect to control, #p< 0.05, ##p< 0.005 with respect to HIV-1 Tat and cotransfected group (PC stands for plasmid control, and PC+PC is used as plasmid control in cotransfected cells)