FIGURE 2.

Increased TLR signaling in αv-deficient Tlr7.1 tg B cells. (A) Sorted transitional (T1), MZ, and Fo B cells from Tlr7.tg control and αv-CD19.Tlr7 mice were cultured with 5 μg/ml TLR7 ligand R848 for 0–120 min. Nuclear NF-κB (p65) localization was measured by Western blot, and LSD1 is shown as nuclear loading control. (B and C) Cytokine (B) and IgG2c (C) levels in supernatants of cultured B cell subpopulations after stimulation with R848 for 24 h [cytokines (B)] or 4 d [IgG2c (C)]. Data shown are mean ± SD of replicate cultures from one experiment. The p values <0.05 (Student t test) for comparisons between control Tlr7.tg and αv-CD19.Tlr7 mice are shown (*p < 0.05). Similar results were seen in at least three independent experiments.