FIGURE 5.

Abs that target the NMJ were reduced in high-dose anti-survivin–treated EAMG animals. TA muscle was sectioned and stained for mouse-specific IgG and α-BTX, which labeled AChR. Images were taken on a Carl Zeiss Cell Observer Spinning Disk Confocal Microscope and analyzed for fluorescent intensity by Zen software. (A) Representative images of Alexa 488–anti-mouse IgG and Alexa 594–α-BTX for EAMG PBS-treated and EAMG 2C2-treated (high- and low-dose) mice. (B) Fluorescent intensity measurements of Alexa 488–anti-mouse IgG. (C) Fluorescent intensity measurements of Alexa 594–α-BTX. Data were presented as mean ± SD. Results were representative of two independent experiments of 30 NMJs analyzed per animal. n = 9 for all groups. Statistical comparisons were performed by Student t test analysis. Statistical p values < 0.05 were considered significant.