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. 2020 Sep 7;11:1048. doi: 10.3389/fphys.2020.01048

Figure 6.

Figure 6

Chronic alcohol and liver clock disruption alter abundance and phosphorylation state of select fatty acid metabolism proteins. Diurnal protein abundances of total acetyl CoA carboxylase (Total ACC; A), phosphorylated acetyl CoA carboxylase (p-ACC; B), malonyl-CoA decarboxylase (MCD; C), and fatty acid synthase (FASN; D) were determined by western blotting using liver homogenates of control-fed (black) and alcohol-fed (red) Bmal1 Flox/Flox (Fl/Fl) and control-fed (purple) and alcohol-fed (green) Bmal1 liver-specific knockout (LKO) mice from ZT 3, 7, 11, 15, 19, and 23 (ZT 0: lights on/inactive period, ZT 12: lights off/active period). Protein abundances were detected using the LI-COR Odyssey® digital imaging system. A representative image is presented for each protein of interest showing a full time course. The bar graphs indicate the average protein abundance calculated over the 24 h day normalized to β-actin, and levels are displayed as a fold-change from the control-fed Fl/Fl group. Data are shown as mean ± SEM n = 3–4 mice/genotype/diet. Significant two-factor ANOVA results are included in graphs. Letters indicate statistically significant differences (p < 0.05) for comparisons to control-fed Fl/Fl mice (a), alcohol-fed Fl/Fl mice (b), and control-fed LKO mice (c).