Table 1.
Different types of CRISPR/Cas proteins that have been shown to be effective for plant genome editing
| Cas protein | Source | Nucleic acid cleavage | PAM sequence | Reference |
|---|---|---|---|---|
| spCas9 | Streptococcus pyogenes | DNA blunt ends | NGG | 40 |
| FnCpf1 (Cas12a) | Francisella novicida | DNA staggered ends | TTTN | 7 |
| LbCpf1 (Cas12a) | Lachnospiraceae bacterium | DNA staggered ends | TTTN | 7 |
| AsCpf1 (Cas12a) | Acidaminococcus sp. | DNA staggered ends | TTTN | 41 |
| AaCas12b (C2c1) | Alicyclobacillus acidiphilus | DNA staggered ends | TTN | 42 |
| LwaCas13 (C2c2) | Leptotrichia wadei | Single stranded RNA | 43 |