Figure 2.

AP770 is detectable only in platelets, not in other blood cell types. Human peripheral blood mononuclear cells, isolated from blood samples, were analyzed by flow cytometry. A–C, cell surface APP or APP770 expression was evaluated in CD13⁺ peripheral myeloid cells (A), CD3⁺ T-lymphocytes (B), and CD19⁺ B-lymphocytes (C) using anti-APP (22C11) and anti-hAPP770 antibodies. (D) Leukocytes, T cells, B cells, monocytes, and platelets from human blood samples were lysed in T-PER reagent containing protease inhibitor mixture and were used for Western blotting analysis with antibodies against CD45, CD3, CD19, CD14, CD42b, CD40L, APP770, APP (22C11), and β-actin. E, human peripheral lymphoid cells, isolated from blood samples, were incubated in the presence or absence of cell stimulation mixture for 5 h and then were subjected to flow cytometry analysis with anti-CD40L and APP770 antibodies. Cont, control.