Skip to main content
. 2020 Sep 18;88(10):e00270-20. doi: 10.1128/IAI.00270-20

FIG 4.

FIG 4

Identification of additional factors required for vaginal colonization and persistence by transposon mutant library screen. (A) Schematic representing experimental approach. C57BL/6 mice were treated with 17β-estradiol prior to inoculation with the OG1RF Tn library in triplicate groups or WT OG1RF. The vaginal lumen was swabbed, and the number of CFU was enumerated daily. DNA from recovered OG1RF Tn mutants was sequenced on days 1, 5, and 8. (B) CFU recovered from vaginal swabs of triplicate groups of mice colonized with OG1RF Tn mutagenesis library (T1, T2, and T3) and OG1RF WT (n = 5 mice per group). (C, D, and E) Volcano plots depicting underrepresented and overrepresented mutants in vivo compared to culture on day 1 (C), day 5 (D), and day 8 (E) postinoculation. (C) A total of 667 underrepresented genes and 21 overrepresented genes in vivo compared to culture. (D) A total of 404 underrepresented genes and 6 overrepresented genes in vivo compared to culture. (E) A total of 507 underrepresented genes and 3 overrepresented genes in vivo compared to culture. Colored dots represent mutants of interest. Pink, ebpC Tn mutant; orange, eut Tn mutants; green, T7SS gene Tn mutants. The black solid line represents the cutoff for statistical significance.