FIG 1.

Cdt holotoxin treatment induces a CdtB-Rab5a-cellugyrin association in human colonic epithelial cells. Primary human colonic epithelial cells were treated with Cdt holotoxin (labeled as “CdtB,” 2 μg/ml, same for all figures) for 1 h, and CdtB-Rab5a-cellugyrin association (“IP”) and expression (“input”) were tested by the coimmunoprecipitation (co-IP) assay and the Western blotting assay, respectively. “IP: IgA/G” is the control for the co-IP assay. The experiments shown were repeated three times, and similar results were obtained.