FIG 4.

Rab5a overexpression enhances CdtB-induced cytotoxicity in human colonic epithelial cells. Stable primary human colonic epithelial cells, with Rab5a cDNA adenovirus or “Rab5-OE (L1/L2),” as well as vector control cells (“Vec”), were treated with Cdt holotoxin (2 μg/ml) for the applied time, and the expression of the listed genes was tested by qPCR (A) and Western blotting (B); cell viability and cell death were tested by an MTT assay (C) and an LDH medium release assay (D), respectively; cell apoptosis was tested by the annexin V-FACS assay (E and F). *, P < 0.05 versus Ctrl treatment of Vec cells. ^#, P < 0.05 versus CdtB treatment of Vec cells. The experiments reflected in this figure were repeated four times, and similar results were obtained.