FIG 2.

Southern blot analysis of E. chaffeensis Himar1 mutants. Genomic DNA from Himar1 transposon E. chaffeensis mutants was assessed by DNA blot analysis using a spectinomycin resistance gene (aadA) probe. Panels A to D represent four DNA blot analysis experimental data generated four independent times to locate all insertion mutations within E. chaffeensis genome. Genomic DNAs from the cultured mutants were digested with BglII restriction enzyme (mutants’ codes are identified at the top). Genomic locations for the DNA fragments (identified in Fig. S1) were established by sequence analysis.