TABLE 4.
E. chaffeensis gene disruption mutants cleared from the canine host
| Mutant no. | Mutant code | Gene no. | Protein identifier |
|---|---|---|---|
| 1 | A1-1a | ECH_0113 | Hypothetical protein |
| 3 | D4-1 | ECH_0242 | Hypothetical protein |
| 4 | s33 E5 | ECH_0251 | Hypothetical protein |
| 5 | D3-2 | ECH_0368 | Dioxygenase family protein (oxidoreductase) |
| 6 | s34 C8 | ECH_0445 | Queuine tRNA-ribosyltransferase (protein synthesis) |
| 7 | C1-1 | ECH_0475 | Signal recognition particle protein (protein synthesis) |
| 8 | s33 C7 | ECH_0525 | Hypothetical protein |
| 10 | B5-1 | ECH_0600 | Hypothetical protein |
| 11 | D3-1 | ECH_0614 | Hypothetical protein |
| 14 | C2-1 | ECH_0666 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (biotin biosynthesis) |
| 15 | A4-1 | ECH_0669 | 3-Oxoacyl-(acyl-carrier-protein) reductase (fatty acid biosynthesis) |
| 16 | s34 A2 | ECH_0843 | Recombination protein RecR (DNA repair) |
| 20 | s34 D8 | ECH_1110 | Dethiobiotin synthetase (biotin biosynthesis) |
| 21 | C3-2 | ECH_1127 | Major outer membrane protein OMP-1V/OMP-6 (immunogenic outer membrane protein) |
| 22 | B6-1 | ECH_1144 | Major outer membrane protein P28-1/OMP-20 (immunogenic outer membrane protein) |
| 23 | C2-3 | ECH_0039 | 120-kDa immunodominant surface protein (immunogenic outer membrane protein) |
| 24 | A4-3 | ECH_0561 | AcrB/AcrD/AcrF family protein (MDR efflux protein) |
| 25 | D4-3 | ECH_0837 | tRNA-i(6)A37 modification enzyme MiaB (metal ion binding/tRNA modification) |
| 27 | TR31 | ECH_1144 | Major outer membrane protein P28-1/OMP-20 (immunogenic outer membrane protein) |
| 28 | D1-4 | ECH_0104 | Hypothetical protein |
| 29 | D5-4 | ECH_0329 | Hypothetical protein |
| 30 | D4-4b | ECH_0666 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (biotin biosynthesis) |
| 31 | D3-4 | ECH_0866 | Hypothetical protein |
a
Tested positive by xenodiagnosis.
b
D4-4 tested positive only in tissue samples of two animals on day 56.