Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 21;10:325. doi: 10.1038/s41398-020-01000-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Mean (±SEM) GFAP-positive cell immunoreactivity in the dentate gyrus (Effect of exposure: F[3,138] = 15.7, p < 0.001; Effect of region: F[2,138] = 2.1, p = 0.15; Interaction: F[3,138] = 0.9, p = 0.45). b Representative photomicrograph of the dorsal dentate gyrus from a representative control stained for GFAP (×10 magnification; scale bar = 100 μm) and close-up representative photomicrographs for each experimental group (×20 magnification; scale bar = 50 μm). c mean (±SEM) GFAP-positive cell density in the dentate gyrus stratified by GFAP cell type and dentate gyrus region (Effect of exposure (a): F[1,168] = 6.9, p = 0.01; Effect of region (b): F[2,168] = 3.6, p = 0.06; Effect of cell type (c): F[1,168] = 350.8, p < 0.001. Interactions: (a × b) F[1,168] = 0.03, p = 0.87; (a × c) F[1,168] = 5.2, p = 0.03; (b × c) F[1,168] = 2.2, p = .15; (a × b × c) F[1,168] = 0.02, p = 0.88). d Representative photomicrographs of a radial glial cell (GFAP+/SOX2+) and astrocyte (GFAP+/SOX2−) taken from socially isolated animals (×40 magnification; scale bar = 40 μm) and representative maximum intensity 2D z-stack projections of ten confocal images taken from socially isolated and control animals (×20 magnification; scale bar = 50 μm). e Mean (±SEM) s100β-positive cell density in the dentate gyrus (Effect of exposure: F[1,84] = 24.0, p < 0.001; Effect of region: F[2,84] = 0.2, p = 0.64; Interaction: F[1,84] = 0.3, p = 0.61). f Representative photomicrographs of s100β-positive cells in the ventral dentate gyrus of controls and socially isolated mice (×40 magnification; scale bar = 50 μm). *p < 0.05; **p < 0.01; ***p < 0.001 (adjusted p-values). Analyses: two-way ANOVA with Bonferroni post hoc comparison or three-way factorial ANOVA with Bonferroni post hoc comparison.