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. 2020 Aug 13;40(10):2454–2467. doi: 10.1161/ATVBAHA.120.315028

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© 2020 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Angiogenesis following femoral artery excision occurs exclusively in injured/regenerating (Regen) muscle zones. A, Contingency table relating the presence of angiogenesis with histologically defined muscle zones. Data from a total of 87 zones from 9 C57BL/6J mice subjected to femoral artery excision and harvested 10 d later are depicted. P<0.0001. B, Micrographs of gastrocnemius muscle sections from a C57BL/6J mouse subjected to hind limb ischemia and harvested 10 d later, depicting the transition from injured to noninjured muscle. Top, Capillary content, based on immunostaining for CD (cluster of differentiation) 31 (green) with 4′,6-diamidino-2-phenylindole (DAPI) nuclear counterstain (blue). Bottom, Near-adjacent section stained with hematoxylin and eosin, illustrating the abrupt transition from centralized to peripheral myofiber nuclei. C, Capillary densities in defined muscle zones of the injured gastrocnemius muscle. *P<0.0001. D, Fluorescence micrographs of regions in the gastrocnemius muscle 10 d after surgery, immunostained for CD31 (green) and Ki-67 (pink). Nuclei are visualized with DAPI. Bord indicates border zone; Cont, control; and Uninj, uninjured zone.