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. 2020 Aug 13;40(10):2376–2390. doi: 10.1161/ATVBAHA.120.314284

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© 2020 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 2. — Effects of CORM-A1 on mitochondrial respiration and glycolysis in parallel to aggregation in activated platelets. Oxygen consumption rate (OCR; A and C) and extracellular acidification rate (ECAR; B and D) measurements of human washed platelets (WP) treated with PBS (control) or CORM-A1 (A1; 10–300 μmol/L) followed by the addition of thrombin (0.1 U/mL) and further sequential addition of FCCP (carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone)/pyruvate (0.3 μmol/L/1 mmol/L) and rotenone/antymycin A (R/A; 0.5/0.5 μmol/L; A and B) or oligomycin (1 μg/mL) and R/A (C and D). Bioenergetic parameters of mitochondrial respiration and glycolysis (E) were calculated as described in the Data Supplement. Data represent the means±SD of 3 independent experiments; n=4–8 replicates in each experiment. F, Concentration of lactate extruded from platelets (WP) treated with CORM-A1 (0, 100, 300, 1000 µmol/L; CONT) or CORM-A1 and thrombin (0.1 U/mL; Thr); data represent the means±SD of 4 independent experiments; n=2 replicates in each experiment. G, Aggregation of platelets (WP) treated with CORM-A1 (10, 30, 100, 300 µmol/L) and activated with thrombin (0.5 U/mL) as compared with control; data represent the means±SD of 4 independent experiments; n=2 replicates in each experiment. *P<0.05, #P<0.01, $P<0.001, &P<0.0001 as compared with control group.