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miR-19a-3p regulates the osteogenic differentiation in BMSCs. (A) miR-19a-3p expression in the BMSCs during osteogenic differentiation. (B) The antagomiR-19a-3p or agomiR-19a-3p were used to silence or overexpress miR-19a-3p in BMSCs. (C, D) Alizarin Red staining revealed the role of miR-19a-3p on the osteogenic differentiation of BMSCs. Scale bars: 100 μm. (E) ALP activity, (F) osteocalcin secretion, (G) RUNX2 and H, osterix expression in BMSCs. Data shown as mean ± SD. *p <0.05.
