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. 2020 Oct 1;11(5):1058–1068. doi: 10.14336/AD.2019.0724

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copyright: © 2020 Chen et al.

this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — miR-19a-3p regulated Hoxa5 expression by targeting the 3’UTR of Hoxa5 directly. (A) the predict binding role of miR-19a-3p on 3′-UTR of Hoxa5. (B) luciferase reporter assay revealed the target role of miR-19a-3p on Hoxa5. (C) miR-19a-3p regulated Hoxa5 expression. (D) Hoxa5 expression was increased in BMSCs of the aging mice. (E, F) The role of Hoxa5 and miR-19a-3p on ALP activity of BMSCs. (G, F) The role of Hoxa5 and miR-19a-3p on osteocalcin secretion of BMSCs. Data shown as mean ± SD. *p <0.05.