Table 1.
The primers.
| Forward 5’-3’ | Reverse 5’-3’ | |
|---|---|---|
| β-actin | CTGTCCCTGTATGCCTCTG | TGATGTCACGCACGATTT |
| Runx2 | ACTTCCTGTGCTCCGTGCTG | TCGTTGAACCTGGCTACTTGG |
| Hoxa5 | CTCATTTTGCGGTCGCTATCC | ATCCATGCCATTGTAGCCGTA |
| Osterix | ACCAGGTCCAGGCAACAC | GCAAAGTCAGATGGGTAAGTAG |
| miR-19a-3p | CTGGAGTGTGCAAATCTATGC | GTGCAGGGTCCGAGGT |
| LncRNA Xist | AATGGAACGGGCTGAGTTTTAG | TCATCCGCTTGCGTTCATAG |
| U6 | GCGCGTCGTGAAGCGTTC | GTGCAGGGTCCGAGGT |
| Antagomir-19a-3p | TCAGTTTTGCATAGATTTGCACA | |
| antagomir NC | CAGUACUUUUGUGUAGUACAA | |
| agomir-19a-3p | UGUGCAAAUCUAUGCAAAACUGA | |
| pGL3-HOXA5 | GGGGTACC GCATCTGAGCG | CCGCTCGAG GCTGATCACAGTT |
| pGL3-Xist | GGGGTACCCGGCTTGCTCCA | CCGCTCGAG TTCAAAACAAAGCA |
| pcDNA3.1-HOXA5 | GGGGTACC ATGAGCTCTTATT | CCGCTCGAG TCAGGGGCGGAA |
| pcDNA3.1-Xist | GGGGTACCCGGCTTGCTCCA | CCGCTCGAG TTCAAAACAAAGCA |
| siHOXA5 | GCACATTAGTCACGACAAT | |
| si_control | GCATTAGTCACGACCAAAT | |
| siXist | GCACACATCTCATTCCATT |