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. 2020 Sep 21;15(9):e0239495. doi: 10.1371/journal.pone.0239495

Table 1. List of RNA-Seq datasets used in this study.

ID Description Data Type Source
HCA CB Umbilical cord blood PBMCs from the Human Cell Atlas; in total ~254,000 cells from 8 patients. Single cell, 10x genomics, UMI counts. Li et al [38], Rozenblatt-Rosen et al [39]. The data can be downloaded from https://data.humancellatlas.org/, Census of immune cells.
LC ~39,000 cells from the tumor microenvironment of lung cancers and ~13,000 cells from adjacent healthy tissue. The cells originate from 5 patients. Single cell, 10x genomics, UMI counts. Lambrechts et al [40]. The data is available in in ArrayExpress under accessions E-MTAB-6149 and E-MTAB-6653.
PBMC68k ~68,000 PBMCs from blood, one patient. Single cell, 10x genomics, UMI counts. Zheng G.X.Y. et al [4]. The data is available at 10x Genomics’ home page.
B10k ~10,000 FACS-sorted CD19+ B cells from blood, one patient. Single cell, 10x genomics, UMI counts. Zheng G.X.Y. et al [4]. The data is available at 10x Genomics’ home page.
CD4TMEM ~10,000 FACS-sorted CD4+/CD45RO+ Memory T Cells, one patient. Single cell, 10x genomics, UMI counts. Zheng G.X.Y. et al [4]. The data is available at 10x Genomics’ home page.
TCD8 ~10,000 FACS-sorted CD8+ T cells from the blood of a single patient. Single cell, 10x genomics, UMI counts. Chen et al [41]. The data is available for download on GEO data repository, accession number GSE 112845.
MEL ~4,600 cells from the tumor microenvironment of Melanoma, 19 patients. Single cell, SMART-Seq2, TPM Tirosh et al [42]. The data is available for download on GEO data repository, accession number GSE 72056.
EVAL Dataset produced for evaluating the performance of existing single-cell technologies. Data from mouse brain, PBMC and cell lines. Data includes 7 single-cell technologies and bulk, all performed on the same samples. Single cell data from 7 different technologies and corresponding bulk samples, counts/ UMI counts/ TPM Ding et al [26]. The data is available for download at the Single Cell Portal, id SCP425.
BULK 1 In total 6 bulk samples from B cells of varying origin. Bulk RNA-Seq, FASTQ files The ENCODE Consortium [43, 44], Gingeras. The samples can be downloaded individually from ENCODE.
BULK 2 In total 7 bulk samples from B cells (1) and T cells (6) of varying origin. Bulk RNA-Seq, FASTQ files The ENCODE Consortium [43, 44], Stamatoyannopoulos and Weng. The samples can be downloaded individually from ENCODE.
BULK 3 In total 12 bulk samples from B cells (6) and T cells (6) of varying origin. Bulk RNA-Seq, FASTQ files The functional annotation of the mammalian genome 5 (FANTOM5) [45, 46]. The data can be downloaded from FANTOM5.
BULK 4 In total 39 bulk samples from B cells (16) and T cells (23) of varying origin. Bulk RNA-Seq, FASTQ files The BLUEPRINT Epigenome Project [47]. The samples can be downloaded individually from BLUEPRINT.
BULK 5 In total 10 PBMC bulk samples from B cells (5) and T cells (5). Bulk RNA-Seq, RPKM/counts Pabst et al [48], GSE 51984.