Skip to main content
. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Fly (Austin). 2010 Apr 18;4(2):95–103. doi: 10.4161/fly.4.2.11861

Figure 2.

Figure 2

Genetic scheme for the INAD-GFP localization screen. The genetic scheme used to generate INAD-GFP expressing mutant lines for screening. Third chromosome eMs mutagenized males from the Zuker collection (* indicates the mutation on the third chromosome) and P(inaD-GFP); bw; DTS4 D/TM6B females were crossed at 29°c for five days. The non-permissive temperature of 29°c was used to eliminate all progeny containing DTS4 D in the F1 generation. All viable F1 males and females were inbred at 25°c. DTS4 escapers (not shown in the scheme) were identified by the presence of the dominant Dichete (D) mutation. F2 homozygous mutant flies (bw; st*) were identified by the presence of white eyes and screened for INAD-GFP localization.