Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Drosophila melanogaster) | btl-GAL4 | Shiga et al., 1996 | FLYB: FBtp0001208 | This line was a gift from Dr.Shigeo Hayashi |
Genetic reagent (Drosophila melanogaster) | UAS-Chk1RNAi | VDRC | 110076 | |
Genetic reagent (Drosophila melanogaster) | UAS-TCFRNAi | VDRC | 3014 | |
Genetic reagent (Drosophila melanogaster) | UAS-Chk1 |
Kizhedathu et al., 2018 and This paper |
FLYB: FBgn0261278 | Developed using clone from DGRC: UFO05423 |
Antibody | Phospho-Chk1 (Ser345) | CST | 2348 | Rabbit monoclonal antibody (1:200) |
Commercial assay or kit | Tyramide signal amplification system | Thermo Fisher Scientific | B40922 |