REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Akt	Cell Signaling Technology	Cat# 9272, RRID:AB_329827
Mouse monoclonal anti-Akt p-S473	Cell Signaling Technology	Cat# 12694, RRID:AB_2797994
Rabbit polyclonal anti-ATG13	Sigma-Aldrich	Cat# SAB4200100, RRID:AB_10602787
Sheep polyclonal anti-ATG13	MRC PPU Reagents & Services	Cat# S777C
Rabbit polyclonal anti-ATG13 p-S318	Novus	Cat# NBP2-19127
Rabbit monoclonal anti-BRAF	Thermo Fisher Scientific	Cat# 702187, RRID:AB_2633065
Rabbit polyclonal anti-CK1α	Bethyl	Cat# A301-991A RRID:AB_1576501
Sheep polyclonal anti-CK1α	MRC PPU Reagents & Services	Cat# SA527
Rabbit polyclonal anti-CUL2	Invitrogen	Cat# 51-1800, RRID:AB_2533898
Rabbit polyclonal anti-EGFR	Santa Cruz Biotechnology	Cat# sc-03, RRID:AB_631420
Rabbit monoclonal anti-EGFR p-Y1068	Cell Signaling Technology	Cat# 3777, RRID:AB_2096270
Rabbit polyclonal anti- ERK1/2	Cell Signaling Technology	Cat# 9102, RRID:AB_330744
Mouse monoclonal anti-ERK1/2 p-T202/Y204	Cell Signaling Technology	Cat# 9106, RRID:AB_331768
Sheep polyclonal anti-FAM83D	MRC PPU Reagents & Services	Cat# SA102
Rabbit polyclonal anti-FIP200	Proteintech	Cat# 17250-1-AP, RRID:AB_10666428
Mouse monoclonal HRP-conjugated anti-FLAG	Sigma-Aldrich	Cat# A8592, RRID:AB_439702
Mouse monoclonal anti-FLAG	Sigma-Aldrich	Cat# F1804, RRID:AB_262044
Rabbit monoclonal anti-GAPDH	Cell Signaling Technology	Cat# 2118, RRID:AB_561053
Sheep polyclonal anti-GFP	MRC PPU Reagents & Services	Cat# S268B
Rabbit polyclonal anti-HaloTag	Promega	Cat# G9281, RRID:AB_713650
Mouse monoclonal anti-HIF1α	BD Biosciences	Cat# 610959, RRID:AB_398272
Sheep polyclonal anti-LC3	MRC PPU Reagents & Services	Cat# S400D
Rabbit monoclonal pan-RAS	Abcam	Cat# ab206969
Sheep polyclonal anti-SGK3	MRC PPU Reagents & Services	Cat# S848D
Rat monoclonal anti-α-tubulin	Thermo Fisher Scientific	Cat# MA1-80189, RRID:AB_2210200
Mouse monoclonal anti-mono- and poly-ubiquitinylated Conjugates	Enzo Life Sciences	Cat# BML-PW8810, RRID:AB_10541840
Rabbit monoclonal anti-ULK1	Cell Signaling Technology	Cat# 8054, RRID:AB_11178668
Rabbit polyclonal anti-ULK1 p-S757	Cell Signaling Technology	Cat# 6888, RRID:AB_10829226
Goat anti-rabbit IgG HRP-conjugated	Cell Signaling Technology	Cat# 7074, RRID:AB_2099233
Rabbit anti-sheep IgG HRP-conjugated	Thermo Fisher Scientific	Cat# 31480, RRID:AB_228457
Goat anti-rat IgG HRP-conjugated	Thermo Fisher Scientific	Cat# 62-9520, RRID:AB_2533965
Goat anti-mouse IgG HRP-conjugated	Thermo Fisher Scientific	Cat# 31430, RRID:AB_228307
Goat-anti-mouse IgG Alexa-Fluor 647	Thermo Fisher Scientific	Cat# A-21235
Chemicals, Peptides, and Recombinant Proteins
HaloPROTAC-E	Tovell et al. 2019a	N/A
SGK3-PROTAC1	Tovell et al. 2019b	N/A
VH298	Frost et al. 2016	N/A
MLN4924	Active Biochem	Cat# A-1139
MG132	Abcam	Cat# Ab141003
Bafilomycin-A1	Enzo Life Sciences	Cat# BML-CM110
MRT68921	MRC PPU Reagents & Services	N/A
SBI-0206965	Sigma-Aldrich	Cat# SML1540
S-trityl-L-cysteine (STLC)	Sigma-Aldrich	Cat# 164739
PEI MAX – Transfection Grade Linear PEI Hydrochloride MW 40,000	Polysciences	Cat# 24765
Polybrene (Hexadimethrine bromide)	Sigma-Aldrich	Cat# 107689
GFP-Trap-Agarose	Chromotek	Cat# GTA-20
Anti-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat# A2220
Immobilon Western Chemiluminescent HRP Substrate	Merck	Cat# WBKLS0500
ProLong™ Gold Antifade Mountant with DAPI	Life Technologies	Cat# P36935
Deposited Data
Data obtained in this study	This paper	https://data.mendeley.com/datasets/xjnf2sr577/draft?a=4dd608f3-a50c-42af-bd0f-2d470e6b0ef0
Experimental Models: Cell Lines
A549	ATCC	Cat# CCL-185
ARPE-19	ATCC	Cat# CRL-2302
ARPE-19 ULK1GFP/GFP	This paper	N/A
HEK293	ATCC	Cat# CRL-1573
HEK293 SGK3Halo/-	Tovell et al. 2019a	N/A
HEK293 SGK3GFP/GFP	Malik et al. 2018	N/A
HEK293-FT	Invitrogen	Cat# R70007
U2OS	ATCC	Cat# HTB-96
U2OS FAM83DGFP/GFP	Fulcher et al. 2019	N/A
U2OS FAM83DGFP/GFPCSNK1A1mCh/mCh	Fulcher et al. 2019	N/A
Recombinant DNA
pCMV-gag-pol	Cell Biolabs	Cat# RV-111
pCMV-VSV-G	Cell Biolabs	Cat# RV-110
pBabeD-puromycin FLAG-Halo-aGFP6M	This paper; MRC PPU Reagents & Services	Cat# DU57764
pBabeD-puromycin FLAG-aGFP6M-Halo	This paper; MRC PPU Reagents & Services	Cat# DU57765
pBabeD-puromycin FLAG-aGFP6M-HaloD106A	This paper; MRC PPU Reagents & Services	Cat# DU60748
pBabeD-puromycin FLAG-aHRAS	MRC PPU Reagents & Services	Cat# DU57190
pBabeD-puromycin FLAG-Halo-aHRAS	This paper; MRC PPU Reagents & Services	Cat# DU57462
pBabeD-puromycin GFP	MRC PPU Reagents & Services	Cat# DU32961
pBabeD-puromycin U6 ULK1 N-terminal knockin (KI) Sense guide RNA (gRNA)	This paper; MRC PPU Reagents & Services	Cat# DU57396
pX335 ULK1 N-terminal knockin (KI) Antisense guide RNA (gRNA) + Cas9n	This paper; MRC PPU Reagents & Services	Cat# DU57403
pMA-RQ ULK1 N-terminal GFP donor	This paper; MRC PPU Reagents & Services	Cat# DU57856
Software and Algorithms
ImageJ	Schneider et al. 2012	https://imagej.nih.gov/ij/
SoftWoRx	GE Healthcare	N/A
OMERO	Allan et al., 2012	http://openmicroscopy.org/
Graphpad Prism v8	GraphPad Prism Inc	https://www.graphpad.com/scientific-software/prism/
CK1a spindle localisation quantification macro	Fulcher et al. 2019	N/A