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Effect of nicotine administration (0.5 mg/kg i.p.) on the induction of behavioral sensitization. BALB/c mice (n=6/group) were used for a seven days protocol with a nicotine challenge dose on day 11. One-way ANOVA followed by Tukey’s test revealed a significant difference **p<0.01, ***p<0.001, on days 7 and 11 between normal saline and nicotine-treated groups, which confirmed the development of the behavioral sensitization model group.
