Table 1.
Results of the Clinical Chemistry Evaluation.
| Group | ALT | AST | ALP | TBIL | CREA | UREA | GLUC | CHOL | HDL | LDL | BUN | Pi | Ca++ | Na+ | K+ | Cl− | ALB | TPROT | A/G | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (mg/kg bw/day) | [U/L] | [U/L] | [U/L] | [μmol/L] | [μmol/L] | [mmol/L] | [mmol/L] | [mmol/L] | [mmol/L] | [mmol/L] | [mg/dL] | [mmol/L] | [mmol/L] | [mmol/L] | [mmol/L] | [mmol/L] | [g/L] | [g/L] | ||
| Male | ||||||||||||||||||||
| 0 (Control) | Mean | 56.50 | 79.70 | 104.70 | 2.32 | 30.40 | 4.45 | 5.64 | 1.67 | 1.39 | 0.25 | 12.46 | 2.20 | 2.67 | 146.15 | 4.89 | 101.66 | 47.18 | 63.03 | 2.99 |
| (n = 10) | SD | 7.98 | 11.76 | 25.15 | 0.35 | 5.21 | 0.79 | 0.23 | 0.18 | 0.12 | 0.08 | 2.22 | 0.15 | 0.04 | 1.63 | 0.38 | 2.43 | 1.15 | 2.09 | 0.26 |
| 500 | Mean | 50.70 | 82.20 | 104.50 | 2.28 | 30.50 | 4.51 | 5.94 | 1.76 | 1.46 | 0.27 | 12.63 | 2.19 | 2.65 | 146.65 | 4.80 | 101.81 | 46.90 | 62.06 | 3.11 |
| (n = 10) | SD | 9.87 | 13.53 | 27.50 | 0.83 | 4.50 | 0.39 | 0.55 | 0.22 | 0.17 | 0.09 | 1.08 | 0.21 | 0.07 | 2.32 | 0.17 | 2.40 | 2.46 | 1.97 | 0.34 |
| 1000 | Mean | 46.80 | 82.40 | 103.60 | 2.19 | 28.60 | 4.53 | 5.85 | 1.62 | 1.40 | 0.18 | 12.68 | 2.37 | 2.70 | 146.04 | 5.07 | 100.88 | 47.86 | 65.23 | 2.79 |
| (n = 10) | SD | 4.52 | 7.15 | 26.86 | 0.27 | 3.66 | 0.67 | 0.38 | 0.14 | 0.14 | 0.04 | 1.87 | 0.12 | 0.06 | 1.88 | 0.23 | 1.83 | 1.45 | 2.24 | 0.35 |
| SS | ** | * | * | |||||||||||||||||
| 2000 | Mean | 41.00 | 84.30 | 112.70 | 1.97 | 30.50 | 4.61 | 5.97 | 1.81 | 1.56 | 0.21 | 12.91 | 2.34 | 2.69 | 146.42 | 5.04 | 102.35 | 47.88 | 65.55 | 2.80 |
| (n = 10) | SD | 6.96 | 12.34 | 19.03 | 0.40 | 2.84 | 0.74 | 0.63 | 0.25 | 0.24 | 0.05 | 2.06 | 0.14 | 0.06 | 2.30 | 0.40 | 2.55 | 1.48 | 3.87 | 0.54 |
| SS | ** | |||||||||||||||||||
| Test for Significance | DN | NS | NS | NS | NS | NS | NS | NS | NS | DN | NS | DN | NS | NS | NS | NS | NS | NS | NS | |
| Historical Control Range | 26.0–70.0 | 65.0–131.0 | 62.0–209.0 | 0.4–2.5 | 20.0–35.0 | 3.3–8.9 | 4.7–9.2 | 1.4–3.1 | NE | NE | NE | 1.5–2.3 | 2.4–2.9 | 141.2–148.4 | 4.1–5.2 | 96.8–103.2 | 40.1–47.3 | 59.9–70.1 | 1.5–2.6 | |
| Female | ||||||||||||||||||||
| 0 (Control) | Mean | 42.80 | 80.10 | 57.60 | 2.19 | 31.20 | 5.46 | 5.88 | 1.64 | 1.59 | 0.14 | 15.29 | 1.85 | 2.69 | 143.20 | 4.15 | 100.35 | 53.65 | 67.49 | 3.94 |
| (n = 10) | SD | 8.77 | 17.93 | 12.91 | 0.45 | 2.78 | 0.75 | 0.50 | 0.24 | 0.22 | 0.03 | 2.10 | 0.23 | 0.08 | 1.16 | 0.19 | 2.11 | 2.56 | 3.72 | 0.44 |
| 500 | Mean | 41.90 | 80.00 | 57.20 | 2.28 | 32.30 | 5.22 | 5.55 | 1.52 | 1.52 | 0.11 | 14.62 | 1.75 | 2.67 | 143.65 | 4.21 | 100.78 | 54.39 | 67.09 | 4.29 |
| (n = 10) | SD | 8.27 | 12.88 | 17.48 | 0.34 | 4.06 | 0.80 | 0.62 | 0.16 | 0.14 | 0.03 | 2.23 | 0.25 | 0.08 | 2.68 | 0.31 | 2.54 | 3.16 | 4.13 | 0.24 |
| 1000 | Mean | 34.50 | 78.40 | 52.80 | 1.99 | 31.50 | 4.97 | 5.64 | 1.50 | 1.51 | 0.13 | 13.92 | 1.84 | 2.62 | 142.50 | 4.06 | 99.44 | 53.74 | 67.31 | 4.03 |
| (n = 10) | SD | 7.09 | 18.62 | 17.84 | 0.33 | 4.17 | 0.62 | 0.40 | 0.31 | 0.28 | 0.05 | 1.73 | 0.16 | 0.03 | 1.36 | 0.25 | 1.72 | 2.17 | 2.42 | 0.56 |
| SS | * | |||||||||||||||||||
| 2000 | Mean | 35.90 | 81.20 | 57.00 | 1.91 | 32.80 | 5.02 | 6.32 | 1.59 | 1.61 | 0.11 | 14.06 | 1.71 | 2.60 | 143.03 | 4.10 | 101.76 | 53.59 | 67.79 | 3.86 |
| (n = 10) | SD | 10.02 | 16.68 | 21.16 | 0.37 | 2.62 | 0.80 | 0.42 | 0.27 | 0.26 | 0.04 | 2.25 | 0.20 | 0.05 | 1.96 | 0.17 | 2.37 | 2.23 | 2.30 | 0.65 |
| SS | * | |||||||||||||||||||
| Test for Significance | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | U | NS | NS | NS | NS | NS | NS | |
| Historical Control Range | 28.0–133.0 | 66.0–249.0 | 22.0–162.0 | 0.5–3.6 | 24.0–40.0 | 3.8–9.5 | 4.0–7.3 | 1.1–2.8 | NE | NE | NE | 0.8–2.1 | 2.4–2.9 | 140.9–146.5 | 3.1–4.6 | 97.6–105.0 | 43.8–57.6 | 56.5–78.9 | 1.7–3.7 | |
Abbreviations: A/G, albumin to globulin ratio; ALB, albumin; ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BUN, blood urea nitrogen; Ca++, calcium; CHOL, cholesterol; Cl−, chloride; CREA, creatinine; DN, Duncan's multiple range test; GLUC, glucose; HDL, high-density lipoprotein; K+, potassium; LDL, low-density lipoprotein; Na+, sodium; NE, laboratory historical control data not yet established—new parameter in accordance with OECD 408 (25 June 2018); NS, Not Significant; Pi, inorganic phosphorous; SD, standard deviation; SS, statistically significant compared to control; TBIL, total bilirubin; TPROT, total protein; U, Mann-Whitney U test versus control.
p < 0.05.
p < 0.01.