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. 2020 Sep 21;10:15389. doi: 10.1038/s41598-020-72409-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ECD on chlamydial pathogenesis after Chlamydia infection in the early active period. (A) Chlamydia infectivity in ECD and control mice (n = 12 per group) infected at ZT15 was determined. Data was analyzed using a two-way repeat measure ANOVA and Tukey post hoc test. (B) ECD mice had periovarian cysts (next to the ovary, as indicated by the white arrow), while infected control mice did not have periovarian cysts (n = 6 per group). (C) Fertility of ECD and control mice infected with C. muridarum at ZT15 was determined by analyzing the number of pups per mouse (n = 6 per group). The data was analysed using a one-way ANOVA and Tukey post hoc test.