Fig. 3. Clonal expansion of CD8 T cells in psoriatic arthritis.

a, b TCR beta-chain V and J-gene usage in PsA blood and synovial fluid generated from 10× 5′ data of patients PSA1505, PSA1607 and PSA1801. V and J segments are represented by arcs, scaled in size to their combined usage by all patients within each sample type, and giving equal weight to each patient. V–J pairings are represented by ribbons. Plot generated using VDJtools (v1.2.1)³⁶. c UMAP of PsA synovial fluid CD4 and CD8 T cells from three patients with clonally expanded cells highlighted in red. Clonally expanded cells in synovial fluid associated most strongly with HLA-DR-high CD8, cycling and ZNF683+ CD8 clusters (odds ratios 12.6, 4.73 and 3.66; adjusted p value 0, 8.7E−19 and 6.1E−64, respectively; two-sided Fisher’s exact test with Bonferroni correction using R stats package; Supplementary Data 7a). d–f Individual clonal expansion across blood and synovial fluid for three PsA patients based on 10× 5′ data. Circles represent CD8 clonotypes, triangles represent CD4 clonotypes. Data points coloured red show significantly expanded clonotypes (adjusted p value ≦ 0.05). Two-sided Fisher’s exact test, Benjamini–Hochberg correction. g Cluster assignment of enriched CD8 clones in PsA synovial fluid from three patients (PSA1505, PSA1607 and PSA1801). h UMAP plot of integrated memory T cells from one donor (PSA1607), including cells from 5′ 10× and SS2 datasets (Supplementary Fig. 8). Synovial CD8 T cells from the clone most enriched in synovial fluid for this patient are highlighted in blue for the 10× data set and red for the SS2 data set. Source data are provided as a Source Data file.