Fig. 2.

Protein expression of ERβ, TLR4 and the downstream molecules myd88 and MMP2 in NSCLC cell lines treated with estrogen and inhibitors. a, b Western blot analysis of ERβ, TLR4, myd88 and MMP2 protein levels in A549 (a) and H1793 (b) cells treated with DMSO (CTRL), E2 (10 nM), E2 + Ful (100 nM), E2 + CLI-095 (100 nM), or E2 + Ful + CLI-095 for 48 h. The combination of fulvestrant and CLI-095 synergistically inhibited the ERβ and TLR4/myd88/MMP2 pathway. c, d Detection of the relative O.D. values of the Western blot analysis. e Immunofluorescence staining of ERβ and TLR4 in A549 cells. Scale bar, 200 μm. f Detection of the mean intensity of fluorescence staining