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. 2020 Sep 8;8:840. doi: 10.3389/fcell.2020.00840

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Copyright © 2020 Wang, Ji, Wang, Qiu, Zhou, Liu, Wan, Lin, Liu, Zhang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Down-regulation of miR-497 expression in tumor tissues of human lung cancer patients. (A) Expression levels of miR-497 in 56 pairs of lung cancer tumor and adjacent normal specimens were analyzed by stem-loop qRT-PCR, and normalized to the levels of U6. The fold changes were obtained by the ratio of miR-497 abundance in cancer tissues to that in the adjacent normal tissues. (B) Relative expression levels of miR-497 in different stages of cancer tissues. (C) qRT-PCR analysis of miR-497 expression in 56 pairs of primary NSCLC tissues and their corresponding lymph node metastases. (D) miR-497 expression was decreased in plasma of NSCLC patients. The relative expression levels of miR-497 were detected and normalized to the spiked-in control cel-miR-39 (spiked-in control). Data were presented by mean ± SD. of 3 replicates. *indicated P < 0.05, ** indicated P < 0.01.