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. 2020 Sep 8;8:571501. doi: 10.3389/fcell.2020.571501

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Copyright © 2020 Cirino, Aurigemma, Franzese, Lania, Righelli, Ferrentino, Illingworth, Angelini and Baldini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in chromatin accessibility during differentiation in P19Cl6 cells. (A) Experimental scheme illustrating the three time points tested, T1 (13 h after transfection of siRNA), D1 (24 h after 5Aza addition to the media), and D2 (24 h after addition of DMSO to the media). (B) Quantitative ATAC assays of previously identified TBX1 binding sites associated with the genes indicated. All sites were found to be accessible by ATAC-seq. In all cases, accessibility tends to increase at D2. The negative control locus is located in a gene desert region (see Table 2 for primers sequences). Values are the average of two biological replicates ± standard deviation. (C) Gene expression analysis by quantitative real time PCR of the same genes. Values are the average of two biological replicates ± standard error of the mean.