TABLE 1.
Primer sequences.
| Gene or locus | Primer sequence |
| Tbx1 (65 bp) | FW (65 bp): CTGACCAATAACCTGCTGGATGA RV (65 bp): GGCTGATATCTGTGCATGGAGTT |
| Tbx1 (239 bp) | FW (239 bp): TTTGTGCCCGTAGATGACAA RV (239 bp): AATCGGGGCTGATATCTGTG |
| Pecam1 | FW: TGGTTGTCATTGGAGTGGTC RV: TTCTCGCTGTTGGAGTTCAG |
| Unc5b | FW: CAATCCAGCCCCACTCAAT RV: GTCCACGCTCCATCCACT |
| Nanog | FW: AAGTACCTCAGCCTCCAGCA RV: GTGCTGAGCCCTTCTGAATC |
| Pou5f1 | FW: TCAGCTTGGGCTAGAGAAGG RV: TGACGGGAACAGAGGGAAAG |
| Nrp1 | FW: CAGTGGCACAGGTGATGACT RV: TCTGATTGGATGGTGCTGTC |
| Gapdh | FW: TGCACCACCAACTGCTTAGC RV: TCTTCTGGGTGGCAGTGATG |
| Bai2 | FW: GCAATGACCTGTTCACCACC RV: GCCTGTCTGCGCCATGTATAG |
| Cdc42bpg | FW: GTAACGTGGCGCAGTTTCTG RV: CCCTCTGCCAATCACCTTCA |
| Brd4 | FW: TCCTCTGAGTCGGAGAGCAC RV: TCCCAGTGTGCCCCTTCTTT |
| Axin2 | FW: TCCGGCTATGTCTTTGCACC RV: TACTCCCCATGCGGTAAGGA |
| Pxn | FW: GGAGAGATGAGCAGTCCGCA RV: CCATCCACTCTCTGTTCCAGG |
| Dusp7 | FW: CCTCCAAGGTGGTTTCAACA RV: GACGAGCTGTCCACGTTAGT |
| Positive Control Gapdh-P (Q-ATAC) | FW: GGGAAGCAGCATTCAGGTCT RV: TGAAATGTGCACGCACCAAG |
| Negative Control (Q-ATAC) | FW: TGCACAACACTGGGCCATTA RV: GTGAGTGAAGGCCATCGGTT |
| Bai2 (Q-ATAC) | FW: CCAGAGCACTTGCTGTCTGA RV: ACAGTAAGAGGGGACAGGCT |
| Cdc42bpg (Q-ATAC) | FW: TCAGTCAGCACTGGAAGCTG RV: TCGGAAGGAACTCCACTCCA |
| Brd4 (Q-ATAC) | FW: TGGGTAGGACGTCACAAACG RV: TCTGCGCCAAATGTCTGACT |
| Axin2 (Q-ATAC) | FW: CACACCCTCAGAGAACCCAC RV: CAGACTATGGCGGCTTTCCA |
| Pxn (Q-ATAC) | FW: GCTGCTGCTTCTGCTTCATC RV: GTGGGTCCTCATTGGTCTGG |
| Dusp7 (Q-ATAC) | FW: CCAGATCCTGCCCTACCTCT RV: GGGTGTGACGTTGAGGATGT |
| gRNA | TGGCCGAGTACACTACCACC |
| Oligo for homologous recombination | GAGGTCTTCTGGTTTACCCTATATT GTATGACCCCCCCTCCCACGATG TTGCCCTAGGTATGCTTTCCATAGC GGTAAGCCTATCCCTAACCCTCTCCT CGGTCTCGATTCTACGTAGAATTCAT GCCCGGACTCGCCGGCTAAGGGCGC ACAGTGGATGAAACAGATTGTGTCT TTCGACAAGCTGAAACTGACCAATA |