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. 2020 Sep 8;8:539485. doi: 10.3389/fcell.2020.539485

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Copyright © 2020 He, Li, Xu, Ru, Huang, Lin and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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γH2AX immunostaining (red) detection in the interphase of mouse zygotes. Nuclei were stained with DAPI (blue). (A) Control group. Scale bar = 20 μm. (B) H₂O₂-treated group. Scale bar = 20 μm. (C) Quantification of the mean fluorescence intensity of γH2AX in both control and H₂O₂-treated zygotes at different stages during interphase. N = 40 zygotes per group at each stage. Results were analyzed by Student’s t-test. ***p < 0.001. Error bars: SD.