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. 2020 Sep 8;8:539485. doi: 10.3389/fcell.2020.539485

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Copyright © 2020 He, Li, Xu, Ru, Huang, Lin and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of AMPK activity affected the development of mouse embryos under mild oxidative stress. CC, Compound C; SBI, SBI-0206965. (A) Representative image of embryos at 24, 48, and 96 hpi, coinciding with the formation of 2-cell embryos, 4-cell embryos, and blastocysts. Scale bars = 100 μm. (B) Statistics of 2- cell, 4- cell and blastocyst formation rate. More than 150 embryos in each experiment were counted (n = 3). Results were analyzed by one-way ANOVA and Tukey’s test. *p < 0.05; **p < 0.01; ***p < 0.001. Error bars: SD.