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. 2020 Sep 21;10:15385. doi: 10.1038/s41598-020-72393-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal fluorescence microscopy analyses of the cellular location of nucleosomes and cGAS in L929 cells. (A) Representative images showing the cellular location of dsDNA, nucleosomes and cGAS after incubation for 12 h. MCC (Manders' colocalization coefficient) is the ratio of FAM signal that overlaps with Cy3 signal to the total signal of FAM. (B) Representative images showing the cellular location of FAM and Cy5 dual labelled 145-nucleosomes and cGAS after incubation for 12 h. FAM was tagged on the 5′ end of one DNA strand and Cy5 was conjugated in histone H3. Cy3 labelled secondary antibody was used for the immunofluorescence of cGAS. Scale bar, 20 μm.