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. 2020 Sep 21;10:15385. doi: 10.1038/s41598-020-72393-w

Figure 5.

Figure 5

Cellular uptake of nucleosomes stimulates the secretion of INFβ and IL-6 through cGAS activation. (A,B) qRT-PCR analyses of IFNβ and IL-6 expression in THP1 cells upon incubation with different types of nucleosomes (20 nM) for 24 h. (C) qRT-PCR analyses of the time-dependent IFNβ expression in THP1 cells upon incubation with different types of nucleosomes (20 nM). (D) qRT-PCR analyses of the concentration-dependent IFNβ expression in THP1 cells upon incubation with different types of nucleosomes for 24 h. (E,F) qRT-PCR analyses of nucleosome-induced IFNβ and IL-6 expression in THP1 cells after cGAS knockdown by siRNAs. After treatment with siRNAs for 24 h, THP1 cells were incubated with 20 nM nucleosomes for 24 h, and secreted IFNβ and IL-6 in the cell culture media were quantified by ELISA. (G,H) qRT-PCR analyses of IFNβ and IL-6 expression in WT and cGAS knockout L929 cells upon incubation with different substrates (20 nM) for 24 h. For all graphs, error bars indicate the mean ± standard deviation of at least triplicate measurements. Statistical significance was determined based on Student’s t-test (ns, p > 0.05; *0.05 > p > 0.01; **0.01 > p > 0.001; ***0.001 > p).