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. 2020 Sep 8;11:2096. doi: 10.3389/fimmu.2020.02096

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Copyright © 2020 Leite Pereira, Jouhault, Marcos Lopez, Cosma, Lambotte, Le Grand, Lehmann and Tchitchek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modified vaccinia virus Ankara (MVA) affects cell surface expression of C-C motif chemokine receptor (CCR5) and C-X-C motif chemokine receptor 4 (CXCR4) in leukocytes of HIV-ART patients and healthy persons. Whole-blood samples of HIV-ART patients (A) and healthy persons (B) were infected with MVA (red boxes) for 16 h or left non-infected (gray boxes). Cells were stained with a panel of 35 cell markers and analyzed with R software as described in the section “Materials and Methods.” CCR5 and CXCR4 expression levels [median signal intensity (MSI)] of each sample are blotted in the respective jitter plot representations. Significant differences (p < 0.05) between samples are indicated by an asterisk.