Figure 4.

Effects of liver IR combined with intraperitoneal administration of aucubin (5 mg/kg, for 10 days) on liver ROS levels in experimental rats. Superoxide anion was expressed by red fluorescence, and nuclei by blue fluorescence; magnification was 400× and scale bar = 100 μm (A). The red fluorescence area was counted by Image Pro Plus 6.0 software analysis (B). Statistical analyses were performed using the Kolmogorov–Smirnov test followed by one-way ANOVA method. All data were expressed as mean ± SD. ^αSignificant difference from the sham operation group at P < 0.05. ^βSignificant difference from the IRI group at P < 0.05.