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. 2020 Sep 8;8:568092. doi: 10.3389/fbioe.2020.568092

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Copyright © 2020 Yoshimoto, Minier, Yang, Imamura, Stocking, Patel, Terada, Hirai and Kamei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pressure-drop method to generate a series of PDMS membrane displacements in a single device. (A) Membrane displacements are inversely proportional to the distance from the inlet due to a pressure drop along with a microfluidic channel. (B,C) The computer simulation for pressure drop was conducted using input pressure (18, 32, and 45 kPa) and output pressure (atmosphere). (D) Displacement measurement of the device with CCD laser displacement camera when 18, 32, and 45 kPa were applied at the inlet. The red plots indicated the conditions used for stretching stimulation. ANOVA with Tukey-Kramer test compared with all displacements of the pressure chambers at 18, 32, and 45 kPa. p-values were summarized in Supplementary Tables S1, S2. Each plot represents the mean ± standard deviation determined from four independent experiments measuring the two chambers in a single device.