TABLE 1.

Primer sequence used for RT-qPCR.

Primer name	Primer sequence	Size (bp)
Aplnr-F	5′-CCACCTGGTGAAGACTCTCTACA-3′	110
Aplnr-R	5′-TGACATAACTGATGCAGGTGC-3′
Pomc-F	5′-AGATTCAAGAGGGAGCTGGA-3′	159
Pomc-R	5′-CTTCTCGGAGGTCATGAAGC-3′
Pdyn-F	5′-CGGAACTCCTCTTGGGGTAT-3′	154
Pdyn-R	5′-TTTGGCAACGGAAAAGAATC-3′
Penk-F	5′-AACAGGATGAGAGCCACTTGC-3′	474
Penk-R	5′-CTTCATCGGAGGGCAGAGACT-3′
Oprm1-F	5′-ATCCTCTCTTCTGCCATTGGT-3′	127
Oprm1-R	5′-TGAAGGCGAAGATGAAGACA-3′
Oprd1-F	5′-AAGTACTTGGCGCTCTGGAA-3′	125
Oprd1-R	5′-GCTCGTCATGTTTGGCATC-3′
Oprk1-F	5′-CCGATACACGAAGATGAAGAC-3′	341
Oprk1-R	5′-GTGCCTCCAAGGACTATCGC-3′
Adcy1-F	5′-CCGGAACATGGACCTCTACTAC-3′	284
Adcy1-R	5′-ATAGGTGGGAGGAGATGGACTG-3′
Adcy2-F	5′-CCTGGGACCAGGTGTCATTC-3′	412
Adcy2-R	5′-CCTGCTTTGGGTCCCTGTAG-3′
Adcy3-F	5′-TACTTCAAAAGGCAGCGCCA-3′	482
Adcy3-R	5′-TTGGCCAGGATCTCCCTCAG-3′
Adcy4-F	5′-TTGACCCAAAGCGGGCAG-3′	248
Adcy4-R	5′-GCACACAGCACAGTTGTCAG-3′
Adcy5-F	5′-ACTTGGCCATCTCTCTGCAC-3′	445
Adcy5-R	5′-TGATTCTCCGCAGCCAACTT-3′
Adcy6-F	5′-GCGGTGAGGGAGAATCACTG-3′	163
Adcy6-R	5′-TCACACCTGTTACCTCACGC-3′
Adcy7-F	5′-GCAGGTAACAGGGTCGGAG-3′	392
Adcy7-R	5′-AGGTCCTCAGCTCTTTGCAC-3′
Adcy8-F	5′-TTGCGGAGTGGCGATAAGTT-3′	482
Adcy8-R	5′-ACAAAGTACTCTGGGTAGGAGC-3′
Adcy9-F	5′-AAGACCAGCACCAAGGCTTC-3′	183
Adcy9-R	5′-GTTCTTGAACCTGAGCGGGA-3′
Bdnf-F	5′-TGGCTGACACTTTTGAGCACGTC-3′	135
Bdnf-R	5′-GCTCCAAAGGCACTTGACTGCTGA-3′
Fos- F	5′-GGTGAAGACCGTGTCAGGAGGCAG-3′	117
Fos-R	5′-GCCATCTTATTCCGTTCCCTTCGG-3′
Creb1-F	5′-TACCCAGGGAGGAGCAATAC-3′	183
Creb1-R	5′-GAGGCAGCTTGAACAACAAC-3′
Dr1-F	5′-TCGGCAGACATGTTGTGAGG-3′	268
Dr1-R	5′-TCTAGGGACACCACTCCCAG-3′
Egr1-F	5′-GAGCACCTGACCACAGAGTC-3′	172
Egr1-R	5′-AAAGGGGTTCAGGCCACAAA-3′
p38α-F	5′-CACAGGGACCTAAAGCCCAG-3′	305
p38α-R	5′-TTCTTCAGAAGCTCAGCCCC-3′
Stat2-F	5′-GTCCTTGAACCGCTTGGAGA-3′	87
Stat2-R	5′-TGCGCCATTTGGACTCTTCT-3′
36B4-F	5′-CGACCTGGAAGTCCAACTAC-3′	109
36B4-R	5′-ATCTGCTGCATCTGCTTG-3′

F, forward; R, reverse.