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. 2020 Sep 8;11:565006. doi: 10.3389/fgene.2020.565006

FIGURE 8.

FIGURE 8

Analysis of PSII and PSI assembly factors proteins and genes, starch and sucrose metabolism pathways marker genes of rice and Arabidopsis in three stages. Heatmap of the expression of photosystems assembly factors in rice (A) and Arabidopsis (B) at different stages. The gene expression levels are shown in different colors indicated by the scale bar. (C) Analysis of photosystems assembly factor proteins in rice and Arabidopsis at different stages. 16 μg total proteins were separated by 12% SDS PAGE. And immunoblots were performed using antibodies as indicated. CBS, Coomassie Blue Staining. (D) The density ratio levels of photosystems assembly factor proteins in (C) and RNA-seq-based gene expression values (FPKM) of relavent genes in rice and Arabidopsis. Data was presented as the means ± SD of three biological replicates. a, b, and c indicate significant differences between the three stages according to Tukey’s test. (E) The relative expression levels of starch and sucrose metabolism pathways marker genes measured by real-time RT-PCR and FPKM of relavent genes in rice and Arabidopsis. Data was presented as means ± SD of three biological replicates. a, b, and c indicate significant differences between the three stages according to Tukey’s test.