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Effect of serum deprivation and LPS treatment on primary astrocytes and microglia between 0-1.5 h. The start of serum deprivation was considered the 0 h time point, and cell morphology was observed every 30 min, for 90 min. LPS was added to the serum-free culture. Scale bar, 50 µm. LPS, lipopolysaccharide.
